Thursday, August 27, 2009

PLant Structure








In this project I was given three steps to describe a plant structure, how does transport in angiosperms works, and the reproduction in angiosperms.




















Plant structure










The roots of a plant are anchor in the soil, which makes it better to absorb mineral, and water to store food. What it also consist of is a palisade mesophyll is a tissue having many packed cylindrical cells. Which differentials the upper epidermis that is a run on of a layer of cells covered by a thick waxy cuticle. This helps the water not get lost, Now the lower epidermis is in a breezier position that why it carries a thinner waxy cuticle. The most talk about tissues of a leave are the xylem and phloem. First the xylem is like a thin straw which water goes upward from the roots to reach the leave this action is called transpiration steam. The heat of the environment gives energy for evaporation of the water from the cell walls. Once the water evaporates it is replaced with water from xylem in the leave. Now the phloem transport all goods like amino acids inside the plants. The process is called active translocation this is, because the sugars and amino acids load into the phloem and parts of the plant that are called sources. Sources are parts of the plant where photosynthesis is occurring. It also involves a stoma which is a piece that enables CO2 for photosynthesis to diffuse. In conclusion main parts of a leave are the blade or lamina















Transport in angiosperms



Angiosperms are flowering plants which forms seeds inside a protective chamber called ovary. Angiosperms are placed in a single division that are split into two classes monocotyledones, and dicotyledones. One of the reasons why the xylem became more specialized during the time period of angiosperm it was for transporting cells during their angiosperm evolution. Cells conduct water in confeirs that are tracheids. Tracheids is in elongated tapered cell that functions in both mechanical support and the movement of water up to the plant. Angiosperm have shorter wider vessels that evolved from tracheids. The xylem of angiosperm is reinforced by a second cell type. The greatest factor in the rise of angiosperm was the evolution of a flower.






Reproduction in angiosperm


A flower is a reproductive structure of an angiosperm, many angiosperm insects and other animals transfer pollen from one flower to female sex organs on to another flower. This makes pollination less random a flower is compressed of many circular ridges sepals, petals, stamens, and carpel. The sepals and petals, I find very interesting see they are sterile and many parts of a flower. What this mainly means is hat they are not involved in reproduction, others are stamens and carpel that are reproductive organs. The stamen is very important to know, being that it has a stalk called filament which is a terminal sac, the anther were pollen is produced. Next a style leads to the ovary which developed into seeds after fertilization. Example running a baseball field you get from one base to the other.
















Friday, February 13, 2009

Transformation Of E.coli with a Green Fluorescent Protein Plasmid

Hypothesis :The efficiency rate for the control would be lower then the
experiment,

Introduction: In this experiment we are using E.Coli that has no antibiotic resistance with supercoiled plasmid that has a gene for antibiotic resistance.During the experiment
bacteria cells will be selected of plasmid by plating them on the agar medium containing ampicillin.

Qualitative Data: While this experiment everything that was going on had the normal Gene that they usually do. However once we added the (GFP) wich then under the u.v light we saw little green dots wich was really amazing we saw the changed immediately. We started to count how many there was it was a total of 71 colonies. More then what other students had we added everything up and that how we got are data.

Quantitative Data: By looking at the quantities data we could see that doing this experiment we work very well as a team we did not seem to have any cross contamination . However their was some mistakes on others students plates wich led us to think they might of cross contaminated and use the same pipet.

Data:
Raw Data




Converted Data








Conclusion: Are hypothesis was correct the rate for the control was lower then
the experiment.

Analysis: By this lab we saw that transformation occurred because of the ampicillin
that we put in to the plate then we left them there overnight. When we finally took
it out of we saw the transformation by putting the room dark and seeing it with a u.v light.

Materials:Gloves, Automatic Micropipet,Thermometer,Ice,Marking pens
Hot plate and long wave U.V

Evaluation: This experiment let Suting and I know more about the transformation on E.Coli. At first I did not understand much of the process , but with her help I got to see
how we did all the bacteria and how to scrape really good to transfer from one to the other.Being that it was my first reall hands on experiment I was really amazed on how we got to see all the little dots. That let us to think we did not have any cross contamination.

Modifications: To make this lab better for everyone some modifications that wil allow
us to make this experiment is making sure your using very different pipets this will not
give you any chances for cross contaminating. Other is work very closely with your partner any questions she will be there to help you. Another is to make sure we write down any usefull information that we know we might need in the near future. Always make sure you put everything that was used in th orange bag.

Safety Considerations: Before starting with the lab make sure the area you and your partner are working is clean you do not want to work in any messy place. Other make sure you are not allergic to any kind of material you might be working with if any concerns ask your professor.
Make sure you wash your hands before and then put gloves on. Any kind of material that already has been used make sure you have a disposable bag for you can throw it in their.

Citations: EDVOTEK- The biotechnology Education Company
www.edvotek.com


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