Transformation Of E.coli with a Green Fluorescent Protein Plasmid

Hypothesis :The efficiency rate for the control would be lower then the
experiment,

Introduction: In this experiment we are using E.Coli that has no antibiotic resistance with supercoiled plasmid that has a gene for antibiotic resistance.During the experiment
bacteria cells will be selected of plasmid by plating them on the agar medium containing ampicillin.

Qualitative Data: While this experiment everything that was going on had the normal Gene that they usually do. However once we added the (GFP) wich then under the u.v light we saw little green dots wich was really amazing we saw the changed immediately. We started to count how many there was it was a total of 71 colonies. More then what other students had we added everything up and that how we got are data.

Quantitative Data: By looking at the quantities data we could see that doing this experiment we work very well as a team we did not seem to have any cross contamination . However their was some mistakes on others students plates wich led us to think they might of cross contaminated and use the same pipet.

Data:
Raw Data




Converted Data








Conclusion: Are hypothesis was correct the rate for the control was lower then
the experiment.

Analysis: By this lab we saw that transformation occurred because of the ampicillin
that we put in to the plate then we left them there overnight. When we finally took
it out of we saw the transformation by putting the room dark and seeing it with a u.v light.

Materials:Gloves, Automatic Micropipet,Thermometer,Ice,Marking pens
Hot plate and long wave U.V

Evaluation: This experiment let Suting and I know more about the transformation on E.Coli. At first I did not understand much of the process , but with her help I got to see
how we did all the bacteria and how to scrape really good to transfer from one to the other.Being that it was my first reall hands on experiment I was really amazed on how we got to see all the little dots. That let us to think we did not have any cross contamination.

Modifications: To make this lab better for everyone some modifications that wil allow
us to make this experiment is making sure your using very different pipets this will not
give you any chances for cross contaminating. Other is work very closely with your partner any questions she will be there to help you. Another is to make sure we write down any usefull information that we know we might need in the near future. Always make sure you put everything that was used in th orange bag.

Safety Considerations: Before starting with the lab make sure the area you and your partner are working is clean you do not want to work in any messy place. Other make sure you are not allergic to any kind of material you might be working with if any concerns ask your professor.
Make sure you wash your hands before and then put gloves on. Any kind of material that already has been used make sure you have a disposable bag for you can throw it in their.

Citations: EDVOTEK- The biotechnology Education Company
www.edvotek.com


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